Monday, May 4th 2020 
 
 12:03-13:00 Registration at the ICVS/EM hall
  
 13:00-17:00 Miniscopes: Past, Present and Future (Dr. Alcino J. Silva)
- Part 1: Introduction to course.
- Break
- Part 2: The uniqueness of miniscopes in neuroscience.

 Tuesday, May 5th 2020 
 09:00-10:40 Imaging principles and Miniscope design
- In vivo recording capabilities and techniques.
- Genetically encoded calcium sensors.
- Principles of fluorescence microscopy.
- The advent of fluorescence micro endoscopy.
- UCLA miniscope design (component overview).
  
 10:40-11:00 Coffee-break
  
 11:00-13:00 5-min presentation by participants
- Participants give a brief presentation regarding their interest in miniscope technology and how this technology might help with their current projects.
  
 13:00-14:00 Lunch
  
 14:00-15:00 5-min presentation by participants (continued)
- Participants give a brief presentation regarding their interest in miniscope technology and how this technology might help with their current projects.
  
 15:00-15:20 Coffee-break
  
 15:20-17:00 Introduction to miniscope assembly
- In depth presentation of miniscope components.
- Where to acquire different components.
- Possible modifications to the current design.
- How to correctly assemble miniscopes and baseplate (hands on session).
Participants will have the opportunity to build and assemble miniscopes.

 Wednesday, May 6th 2020 
 & 
 09:00-13:00 Miniscope assembly (continued)
  
 13:00-14:00 Lunch
  
 14:00-17:00 Lens Implant and baseplate surgeries (Possibly live demonstration)
- Overview of necessary equipment for surgical procedures.
- How to correctly implant GRIN lens and relay lens in different brain regions.
- Base plating, the art of keeping things in place. Practical tutorial for correct implantation of base plate in the skull and how to troubleshoot possible problems.
- How to find the best focal plane for imaging.

 Thursday, May 7th 2020 
 & 
 09:00-13:00 In vivo imaging and behavior
- How to attach and remove miniscopes for data acquisition.
- How to set the best focal plane.
- Hardware and software needed for live acquisition.
- Demonstration of data acquisition.
  
 13:00-14:00 Lunch
  
 14:00-17:00 Image processing and analysis
- General overview of various steps in calcium imaging analysis.
- Image registration.
- Imaging across days, weeks, months.
- Scattering and crosstalk.
- Constrained Non-negative Matrix Factorization.
- Motion correction methods.
- Overlap of activity across sessions.

 Friday, May 8th 2020 
 & 
 09:00-13:00 Student discussion/preparation
- Students are organized in groups of 4-5 and they prepare an original proposal that has miniscope use as its central component. During this activity, students will meet and have the opportunity to interact with instructors in preparing the proposal (2-3 hours).
  
 13:00-14:00 Lunch
  
 14:00-15:30 Student presentations
- Students present their proposals to instructors.
- Students receive feedback from class and instructors.
  
 15:30-16:00 Coffee-break
  
 16:00-17:00 Student presentations (continued)
  
 17:00-18:00 Closing session
- Summary of the material covered during the course.
- Current and future directions of the miniscope technology.



  For the students enrolled in the MSc and PhD programs of the School of Medicine, the course will have 2 mandatory additional weeks: April 27-30 and May 11-15 2020.