In recent years, significant advancements in neuroscience tools have provided researchers with a new in-depth perspective on brain function. Novel anatomical, optical, genetic, electrophysiological, and imaging approaches have opened new possibilities for investigating neuronal circuits.

This hands-on course offers advanced training on various tools used for the analysis of neuronal circuits, including neuronal tracing strategies, fiber photometry, calcium/sensor imaging with miniaturized microscopes, and opto- and chemo-genetics. The course places a strong emphasis on how to design and develop a correct experiment using these tools, and on the appropriate controls, and on the limitations of these methodologies. This approach ensures that participants comprehend the strengths and limitations of each technique and can design, implement, and interpret experiments correctly. By the end of the course, students will have gained the basic skills and knowledge necessary to undertake innovative research projects in circuits neuroscience.

The course is structured into three modules:

Module 1 focuses on the evaluation of neuronal connectivity, encompassing conventional tracing strategies and cutting-edge viral approaches. Participants will also gain hands-on experience in stereotaxic surgeries and brain processing techniques for connectivity analysis.

In Module 2, a critical overview of tools for assessing neuronal activity will be presented. This includes fiber photometry and calcium imaging using 1-photon microscopy. Evaluation of neurotransmitter release using genetically encoded neurotransmitter sensors will also be covered.

Module 3 centers on data analysis techniques for different types of data. Participants will be trained in open-source tools and coding for analyzing calcium imaging data from 1-photon microscopy and neurotransmitter sensor data.

While the modules are interconnected, external participants also have the flexibility to register for individual modules based on their proficiency in specific techniques. Practical classes constitute a significant portion of the course, reflecting its strong emphasis on hands-on training.