This course will be held from May 15th to June 2nd, 2017.
Click here to download the detailed programme.
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Module 1: Cell and Tissue Culture: From Basic Principles to Advanced Techniques |
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Coordinators: António Salgado, Sandra Costa and Luisa Pinto
Goals: Provide a practical and theorical framework on basic and advanced techniques on cells and tissue culture
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| | May 15th 2017: | Introduction to basic aspects of cell and tissue culture |
| | May 16th 2017: | Primary Cell Cultures / Organotypic Cultures
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| | May 17th 2017: | Stem Cells Cultures |
| | May 18th 2017: | Transfection techniques in cell and tissue culture |
| | May 19th 2017: | Animal Cell and Tissue Culture: New Trends |
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Module 2: Gene expression with a focus on qPCR |
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Coordinators: Fernanda Marques, João Sousa
Goals: Provide a practical and theoretical framework on basic aspects of molecular biology, with a focus on gene expression |
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| | May 22nd 2017: | qPCR gene expression analysis: theoretical framework |
| | May 23rd 2017: | Hands-on wet lab: sample RNA extraction and quality analysis / cDNA synthesis
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| | May 24th 2017: | Hands-on wet lab: qPCR preparation / primer design exercises / data analysis |
| | May 25th 2017: | Self-study |
| | May 26th 2017: | Self-study |
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Module 3: Microscopy in Cellular and Molecular Analysis |
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Coordinators: Andreia Castro
Goals: Provide a practical and theorical framework on basic and advanced microscopy techniques in Cellular and Molecular Analysis |
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| | May 29th 2017: | Light and fluorescence microscopy |
| | May 30th 2017: | Optical section and Confocal microscopy
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| | May 31st 2017: | How to build a microscope / Super-resolution microscopy / hands-on on applications |
| | June 1st 2017: | Self-study |
| | June 2nd 2017: | Exam |
