Click here to download the programme.
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Block 1 |
Morning [09:00 - 12:30] |
Afternoon [13:30 - 17:30] |
| e | | |
| April 26th 2017 | Course organization
Brief brain anatomy
Patch clamp introduction
Preparation of recording solutions | Preparation of cutting solutions
Transcardial perfusion
Fresh brain extraction practice |
| e | | |
| | April 27th 2017 | Prepare brain slices | Patch clamp recordings |
| e | | |
| | April 28th 2017 | Prepare brain slices | Patch clamp recordings |
|
Block 2 |
Morning [09:00 - 12:30] |
Afternoon [13:30 - 17:30] |
| e | | |
| May 2nd 2017 | Theory about atlas
Stereotaxic surgeries | Stereotaxic procedures
Surgery |
| | | |
| | May 3rd 2017 | Stereotaxic procedures
Surgery
Injection
Cannula implanatation | Stereotaxic procedures
Surgery
Injection
Cannula implanatation |
| | | |
| | May 4th 2017 | Theory - optogenetics
Behavior optogenetics | Single-cell extracellular electrophysiological recordings
Optogenetic stimulations |
| | | |
| | May 5th 2017 | Electrophysiology whole field | Electorphysiological data analysis |
|
Block 3 |
Morning [09:00 - 12:30] |
Afternoon [13:30 - 17:30] |
| e | | |
| May 8th 2017 | Brain macrodissection (with and without brain slicer) | Electron microscopy (3D reconstruiction, Immunogold, double labeling) |
| | | |
| | May 9th 2017 | Intracellular trafficking and localization
neurosynaptosomes & WB validation | Intracellular trafficking and localization
neurosynaptosomes & WB validation |
| | | |
| | May 10th 2017 | PSD-extrasynaptic fractions
Pre & postsynaptic fractions | PSD-extrasynaptic fractions
Pre & postsynaptic fractions |
| | | |
| | May 11th 2017 | Brain processing - validation & more | Brain processing - validation & more |
| | | |
| | May 12th 2017 | Technical platforms & limitations
Overall discussion | Quizz + evaluation |
Students enrolled in the MSc and PhD programs of the School of Medicine should attend the 3 blocks.
