This hands-on 3 weeks course will provide advanced knowledge and training on multiscale analysis of rodent brain. The focus will be on practicing/performing different techniques/approaches:
- intracranial surgeries in the mouse and in the rat
- stereotaxic injection/dialysis of drugs, viruses, and cells
- optogenetic control of specific cell populations
- electrode implantation: spike and local field potential recording
- brain tissue fixation, sectioning and immunofluorescence analysis
- preparation of acute brain slices
- patch clamp recordings
- analysis of electrophysiological data
- macrodissection of different brain areas with and without the use of brain slicer
- intracellular fractionation and molecular trafficking using both WB
- immunogold analysis & electron microscopy-based 3D reconstruction.
Aiming to bridge across fields of rodent brain analysis and intervention, this course will bring together members of faculty with various expertise and heterogeneous backgrounds in a single hands-on course. Thus, the course will offer a broad-range technical portfolio for multiscale analysis of brain connectome.
This course does not provide credentials for the exercise of medicine for those without appropriate training, recognition and registered at the "Ordem dos Médicos".
