Molecular and Cellular Analysis

Molecular and Cellular Analysis: essential toolkit for in vitro cell cultures, gene expression and microscopy

May 15 - June 2, 2017 ::: 7th Edition
ICVS/EM-UMinho
Molecular and Cellular Analysis: essential toolkit for in vitro cell cultures, gene expression and microscopy

May 15 - June 2, 2017 ::: 7th Edition
ICVS/EM-UMinho
Molecular and Cellular Analysis: essential toolkit for in vitro cell cultures, gene expression and microscopy

May 15 - June 2, 2017 ::: 7th Edition
ICVS/EM-UMinho

Summary
Organization
Faculty
Programme
Evaluation
Registration

Summary

Cell culture techniques, Basic Molecular Biology methods and Advanced Microscopy techniques have become essential tools for basic and applied research, namely in Cellular and Molecular Biology, Physiology, Biotechnology or Medicine. Therefore, this course aims to:

- Improve theoretical and practical fundaments of cell and tissue culture techniques to provide students with the ability to adapt basic cell culture procedures for specific research requirements. Topics covered will include differentiation of primary cells, cloning and selection, cell separation, characterization, transformation and molecular techniques.
- Provide a practical and theoretical framework on basic aspects of molecular biology, with a focus on gene expression
- Introduce Fluorescence and Confocal microscopy as tools in Biomedical Research.

The course has a strong practical component, with a true “hands on” approach, which allows the participants to acquire the necessary skills to apply the above referred techniques to their research programmes.

This course does not provide credentials for the exercise of medicine for those without appropriate training, recognition and registered at the "Ordem dos Médicos".

Organization

Andreia Castro
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

António Salgado
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Fernanda Marques
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

João Sousa
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Luísa Pinto
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Sandra Costa
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Faculty

Ana João Rodrigues
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Andreia Carvalho
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Christian Maibohm
International Iberian Nanotechnology Laboratory (INL), Braga, Portugal

Fábio Teixeira
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Goreti Pinto
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Patrícia Patrício
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

João Sousa
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Nuno Vilas Boas
Olympus

Rita Silva
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Ramiro Almeida
Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra, Portugal

Sofia Serra
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Programme

This course will be held from May 15th to June 2nd, 2017. Click here to download the detailed programme.

	Module 1: Cell and Tissue Culture: From Basic Principles to Advanced Techniques

	Coordinators: António Salgado, Sandra Costa and Luisa Pinto Goals: Provide a practical and theorical framework on basic and advanced techniques on cells and tissue culture

	May 15th 2017:	Introduction to basic aspects of cell and tissue culture
	May 16th 2017:	Primary Cell Cultures / Organotypic Cultures
	May 17th 2017:	Stem Cells Cultures
	May 18th 2017:	Transfection techniques in cell and tissue culture
	May 19th 2017:	Animal Cell and Tissue Culture: New Trends

	Module 2: Gene expression with a focus on qPCR

	Coordinators: Fernanda Marques, João Sousa Goals: Provide a practical and theoretical framework on basic aspects of molecular biology, with a focus on gene expression

	May 22nd 2017:	qPCR gene expression analysis: theoretical framework
	May 23rd 2017:	Hands-on wet lab: sample RNA extraction and quality analysis / cDNA synthesis
	May 24th 2017:	Hands-on wet lab: qPCR preparation / primer design exercises / data analysis
	May 25th 2017:	Self-study
	May 26th 2017:	Self-study

	Module 3: Microscopy in Cellular and Molecular Analysis

	Coordinators: Andreia Castro Goals: Provide a practical and theorical framework on basic and advanced microscopy techniques in Cellular and Molecular Analysis

	May 29th 2017:	Light and fluorescence microscopy
	May 30th 2017:	Optical section and Confocal microscopy
	May 31st 2017:	How to build a microscope / Super-resolution microscopy / hands-on on applications
	June 1st 2017:	Self-study
	June 2nd 2017:	Exam

Evaluation

To get approval in the course students must be present at least in 2/3 of the classes.

Final grade will include the following items (weight of each item in the final grade is included between brackets):
- Final exam (80%)
- Attitude in the classes (20%)

The evaluation is mandatory for students enrolled in the MSc and PhD programs of the School of Medicine and optional for the other participants.

Registration

To register, please fill out the registration form. Within 24/72h, we will send you the confirmation and details for the payment. Please contact us if you do not receive a response in the next 5 days.

Registration deadline: May 9th 2017
Number of vacancies: 20 places
Fees: 150 EUR (only one module) | 350 EUR (full course)

The fee includes all the necessary material for the course and the certificate of attendance.

Click here to access the online form and further details for the registration

EDUCATIONAL SUPPORT

Summary

Organization

Faculty

Programme

Evaluation

Registration

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