T - Lecture ::: P - Laboratory class
This course will be held from February 29th to March 10th 2016 but internal MSc and PhD program students will have an extra week (March 14-18, 2016).
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Monday, February 29th 2016 |
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09:00 - 09:20 |
Registration at the ICVS/ECS hall |
|
09:20 - 10:30 |
Presentation, Introduction and Objectives
T1: Cell and Tissue Culture I: Purpose, Advantages and Applications
[António Salgado]
T2: Cell and Tissue Culture II: Lab Design, Equipment and Aseptic Technique
[António Salgado] |
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11:00 - 11:45 |
T3: Defined Media and Supplements
[António Salgado] |
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12:00 - 13:30 |
Lunch |
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13:30 - 17:30 |
P1: Introduction to cell and tissue culture - Practical lessons |
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18:00 - 18:30 |
Study period |
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09:30 - 10:30 |
T1: Embryonic Development
[Leonor Saúde] |
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11:00 - 12:00 |
T2: Introduction to Stem Cell Biology
[António Salgado] |
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12:00 - 14:00 |
Lunch |
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14:00 - 17:00 |
Introduction to the topics of the written assays [António Salgado / Luísa Pinto] |
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Wednesday, March 2nd 2016 |
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09:30 - 10:00 |
T3: Neuronal cell fate in the adult brain
[Luísa Pinto]
- Identification of neural stem cells and their anatomical locations
- Differentiation potential in vitro and in vivo |
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10:00 - 11:00 |
T4: The Stem Cell Niche in the adult brain
[Luísa Pinto]
- The dominant nature of the niche and the control of stem cells homeostasis
- The role of intrinsic and extrinsic factors |
|
11:00 - 12:00 |
T5: Neural progenitor cells modulation by inflammatory signals: in vs out [João Sousa] |
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12:00 - 13:00 |
Lunch |
|
13:00 - 19:00 |
Neural Stem Cells in the lab[Luísa Pinto]
P2: Isolation of adult neural stem cells
P3: Preparation and observation of neurospheres cultures
P4: Differentiation protocols and immunocytochemistry for the identification of neurons, astrocytes and oligodendrocytes |
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09:30 - 12:00 |
T6: Mesenchymal Stem Cells: Tissue Sources and Characterization [António Salgado]
T7: Hematopoietic Stem Cells
[Ana Fernandes] |
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12:00 - 13:00 |
Lunch |
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13:00 - 19:00 |
P5: Establishment of Primary Cultures Bone Marrow derived MSCs
[António Salgado / Fábio Teixeira] |
|
09:30 - 13:30 |
T8: Introduction to Pluripotent Stem Cells
[Joana Marques]
T9: Genetic Regulatory Networks for the Maintenance of Stem Cell Pluripotency
[José Bragança]
T10: Pluripotent Stem Cells in Cardiac Research and Regeneration
[José Belo] |
|
13:30 - 14:30 |
Lunch |
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14:30 - 17:30 |
P6: Pluripotent Stem Cells - Practical Sessions |
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10:00 - 12:00 |
T11: Cancer Stem Cells
[Bruno Costa] |
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12:00 |
Lunch |
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Afternoon |
Braga Site seeing |
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09:30 - 12:00 |
T12: Motor Neurons and IPScs: Differentiation Cues
[Nuno Lamas]
T13: IPSCs and Bioenergetics: New insight to study pluripotency
[Ricardo Neves] |
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12:00 - 14:00 |
Lunch |
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14:00 - 17:00 |
P7: Assessing motor neuron differentiation in IPSCs |
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Wednesday, March 9th 2016 |
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10:00 - 12:30 |
T14: Microfluidics
[Ramiro Almeida]
P8: Stem Cells and New Tools in
the Lab |
|
12:30 - 14:00 |
Lunch |
|
14:00 - 17:00 |
Stem cells and in vivo animal models
T15: Transplantation Models [Fábio Teixeira, Nuno Silva, Eduardo Gomes] |
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Thursday, March 10th 2016 |
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09:00 - 12:30 |
New tools to Study Stem Cells
T16: Animal Models
[João Peça]
T17: Optogenetics
[Ana João Rodrigues]
T18: Bioreactors
[Fábio Teixeira] |
|
12:30 - 14:00 |
Lunch |
|
14:00 - 17:00 |
P9: In vivo Transplants -
Practical Sessions [António Salgado, Fábio Teixeira,
Luísa Pinto] |
