Hands-on the rodent brain

Summary
Organization
Faculty
Programme
Bibliography
Evaluation
Registration

Summary

This hands-on 3 weeks course will provide advanced knowledge and training on multiscale analysis of rodent brain. The focus will be on practicing/performing different techniques/approaches:

1) intracranial surgeries in the mouse and in the rat
2) stereotaxic injection/dialysis of drugs, viruses, and cells
3) optogenetic control of specific cell populations
4) electrode implantation: spike and local field potential recording
5) brain tissue fixation, sectioning and immunofluorescence analysis
6) preparation of acute brain slices
7) patch clamp recordings
8) analysis of electrophysiological data
9) macrodissection of different brain areas with and without the use of brain slicer
10) intracellular fractionation and molecular trafficking using both WB
11) immunogold analysis & electron microscopy-based 3D reconstruction.

Aiming to bridge across fields of rodent brain analysis and intervention, this course will bring together members of faculty with various expertise and heterogeneous backgrounds in a single hands-on course. Thus, the course will offer a broad-range technical portfolio for multiscale analysis of brain connectome.

This course does not provide credentials for the exercise of medicine for those without appropriate training, recognition and registered at the "Ordem dos Médicos".

Organization

Ioannis Sotiropoulos
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

João Filipe Oliveira
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Faculty

Ana João Rodrigues
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Corrado Cali
King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia

João Peça
Centro de Neurociências e Biologia Celular, Universidade de Coimbra, Portugal

Luísa Pinto
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Patrícia Monteiro
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal

Programme

This course will be held from February 19 to March 9, 2018. Click here to download the detailed programme.

	BLOCK 1 Feb 19-23		BLOCK 2 Feb 26 - Mar 02	BLOCK 3 Mar 05-09

Theory	Brain navigation & connectivity		Brain slice physiology	Brain anatomy & ultrastructure

Brain preparation	Intracranial surgeries Stereotaxic manipulation Electrode/cannula implantation		Preparation of acute brain slices	Brain macrodissection

Approach(es)	Injection/infusion of drugs, virus and cells	Deep-brain recording	Patch-clamp technique	Biochemical/ultra centrifugation	Immunogold + TEM


	Optogenetic manipulation of specific cell population	In vivo electrophysiology recordings	Ex vivo electrophysiology recordings	Intracellular fractionation	3D segmentation & reconstruction

Readout(s)	Specific cell activation/silencing	Specific circuit activation & synchronization	Single cell characterization Synaptic physiology	Intracellular trafficking	Ultrastructural analysis

Students enrolled in the MSc and PhD programs of the School of Medicine should attend the 3 blocks.

Bibliography

Evaluation

To get approval in the course students must be present at least in 2/3 of the classes.

Evaluation will be based on:
a) participation in the practical courses (60%) [20% for each block]
b) a multiple-choice test at the end of the whole course (40%)

The evaluation is mandatory for students enrolled in the MSc and PhD programs of the School of Medicine and optional for the other participants.

Registration

To register, please fill out the registration form. Within 24/72h, we will send you the confirmation and details for the payment. Please contact us if you do not receive a response in the next 5 days.

Individual Block registration is possible!

Number of vacancies 12 places
Fees: 200 EUR (only one block) | 350 EUR (two blocks) | 450 EUR (three blocks)
The fee includes all the necessary material for the course and the certificate of attendance.

Click here to access the online form and further details for the registration

Summary

Organization

Faculty

Programme

Bibliography

Evaluation

Registration

Information

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